Coordinator of Research Team
Maria Teresa Vincenzini
Name of Research Team/Laboratory: cell redox regulation
telephone number: 055-2751238
Brief biographical sketch of the Coordinator
Maria Teresa Vincenzini, born in San Giustino (PG) in 1948, was graduated in Pharmacy (110/110), in Florence (1970), at the Department of Biochemical Sciences.
From 1994 is full professor in Biochemistry (Pharmacy Faculty, Florence, at the Dept. of Biochemical Sciences)and teacher of Biochemistry and Applied Biochemistry in the Course of Degree in Pharmaceutical Chemistry and Technology University of Florence.
From 2013 she is at the Department of Biomedical, Experimental and Clinical Sciences (Biochemistry Section) of the University of Florence.
Professor of the Doctorate in "Biochemical Sciences" and of Specialization School in" Clinical Biochemistry" in the Course of Degree of Medicine and Surgery, Florence University.
Member of the scientific board of the Doctorate in”Biochemical Sciences”
Member of the following Scientific Societies
1) Italian Society of Biochemistry
1) Teresa Iantomasi, researcher,
2) Vladana Domazetovic, postgraduate
Current research interests
-Factors Involved in the defensive mechanisms of neutrophils in Crohn's Disease (CD) patients related to ROS production and intracellular oxidative state. Redox regulation of cytokines and metal proteinases (MMPs) in intestinal myofibroblasts in CD involved in cell adhesion, apoptosis and fibrosis.
-Role of antioxidants and intracellular redox state in the proliferation, differentiation and osteogenic activity of osteoblasts.
-Role of natural antioxidants in induced oxidative stress apoptosis of osteocytes in relation to bone remodeling in osteoporosis and bone regenerative processes.
Current / recent sources of funding
1) MIUR, Bank Foundations
10 best publications of the last 5 years
1 MMPs, ADAMs and Their Natural Inhibitors in Inflammatory Bowel Disease: Involvement of Oxidative Stress. Filippo Fontani, Vladana Domazetovic, Tommaso Marcucci, Maria Teresa Vincenzini and Teresa Iantomasi. Clin Gastroenterol Treat. 2017, 3:039, ISSN: 2469-584X,Volume 3 | Issue 1. Open access.
2. Tumor Necrosis Factor-Alpha Up-Regulates ICAM-1 Expression and Release in Intestinal Myofibroblasts by Redox-Dependent and -Independent Mechanisms. Fontani F, Domazetovic V, Marcucci T, Vincenzini MT, Iantomasi T. J Cell Biochem. 2016 Feb;117(2):370-81. doi: 10.1002/jcb.25279. PMID:26177712
3. Fontani F, Marcucci G, Iantomasi T, Brandi ML, Vincenzini MT.Glutathione, N-acetylcysteine and lipoic acid down-regulate starvation-induced apoptosis, RANKL/OPG ratio and sclerostin in osteocytes: involvement of JNK and ERK1/2 signalling. Calcif Tissue Int. 2015;96:335-346
4. Fontani F, Marcucci T, Picariello L, Tonelli F, Vincenzini MT, Iantomasi T. Redox regulation of MMP-3/TIMP-1 ratio in intestinal myofibroblasts: effect of N-acetylcysteine and curcumin. Exp Cell Res. 2014;323 (1):77-86.
5 Romagnoli C, Marcucci T, Picariello L, Tonelli F, Vincenzini MT, Iantomasi T. Role of N-acetylcysteine and GSH redox system on total and active MMP-2 in intestinal myofibroblasts of Crohn's disease patients. Int J Colorectal Dis. 2013 28(7):915-924.
6 Romagnoli C, Marcucci G, Favilli F, Zonefrati R, Mavilia C, Galli G, Tanini A, Iantomasi T, Brandi ML, Vincenzini MT. Role of GSH/GSSG redox couple in osteogenic activity and osteoclastogenic markers of human osteoblast-like SaOS-2 cells. FEBS J. 2013 Feb;280(3):867-879.
Previous research experiences
Studies on comparative biochemistry and the phylogenetic localization and regulation of a metabolic pathway, the glyoxylate cycle. Other studies regard:
-enzymatic regulation mechanisms, protein catabolism and the influence of vitamin D3 derivatives on some metabolic processes as well as on the activity of metabolite transport systems.
-The characterization and biological role of specific transport systems of GSH and its derivatives in brush-border intestinal membranes of rabbit, rat and men, in synaptosomal membrane of rat cerebral cortex, in human epithelial intestinal cells (I407) and in murine fibroblasts.
-Specific relationships among the antioxidant role of the GSH-system , the acivity of enzymes related to its metabolism and the physiological ageing process and pathologies, such as Crohn's and Alzheimer's diseases and muscular dystrophy.
-The involvement of the GSH-system in mechanisms responsible for radioresistence.
-The role of GSH and the regulator enzyme of its synthesis in the cell oncogenic transformation, in the proliferation of normal and transformed NIH3T3 fibroblasts and in the phosphorylation mechanisms involved in signal mitogenic transduction, in particular on the redox regulation of PDGF receptor.
Main scientific contributions
They regard the data obtained on metabolism and transmembrane transport of GSH, on role of GSH and other antioxidants in the activity and regulation of enzymes and receptors involved in processes such as proliferation, differentiation, apoptosis and inflammation in intestinal cells, miofibroblasts and bone cells.
M. L. Brandi, G. Marcucci, L Picariello
Department of Surgery and Translational Medicine University of Florence.