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SBSC Dipartimento di Scienze Biomediche Sperimentali e Cliniche

Gruppo Pazzagli

Coordinator of Research Team

 Name: Mario Pazzagli

Position: Full Professor in Clinical  Biochemistry; responsible of the SOD “Biochimica Clinica e Molecolare” at the AOU Careggi Hospital

Name of Research Team Laboratory: Clinical Biochemistry and Clinical Molecular Biology

e-mail:m.pazzagli@dfc.unifi.it

telephone number:+39 055 2758411

  

 

 

Brief biographical sketch of the Coordinator (date of birth, degrees and Universities where they have been obtained)

 Date of birth: 30/05/1947, Florence

 Biologist, Ph.D. of the University of  Florence, Full Professor of Clinical Biochemistry and Clinical Molecular Biology, Faculty of Medicine, University of Florence.

 Member of the scientific board of the Doctorate/PhD Program in

 

Scienze Biomediche

 

Member of the following Scientific Societies

 

1) Partner of the Horizon 2020 EU Project: SPIDIA4P


2) Chair of the Working Group "Personalized Laboratory Medicine" of the European Federation of Laboratory Medicine

3) Italian representative of the European Commission for the Clinical Chemist Register (EC4RC);

4)  Member of the Executive Board of the EC4,

5)  Chairman of the EC4-Project Group: European EQA Scheme in Molecular Diagnostics.

6) Member of the IFCC-C-MD commission and Advisor of the CLSI Committee on Molecular Methods.

7) Member of the Executive Board of the European Federation of Clinical Chemistry and Clinical Molecular Biology.

8) SiBioC (Società Italiana di Biochimica Clinica

9) IFCC (International Federation of Clinical Chemistry and Laboratory Medicine)

10) EFLM (European Federation of Laboratory Medicine)


Research Team

1) Pamela Pinzani Associate Professor in Clinical Biochemistry and Clinical Molecular Biology, 15/11/1961

2) Stefania Gelmini PhD,24/05/1965

3) Lisa Simi PhD, 18/09/1972

4) Francesca Salvianti PhD, 30/07/1977

5) Irene Mancini PhD, 21/06/1984

6) Filomena Costanza, PhD Student in Biomedical Sciences 12/08/1988

 

Current research interests 

 

Personalized Medicine

Liquid Biopsy: Circulating Tumor Cell (CTC) and circulating DNA/RNA isolation and characterization Pharmacogenomics

Tumor DNA genotyping

DNA methylation

Gene expression analysis

Standardization: implementation of CEN and ISO technical standards on pre-analytical phase for molecular studies

 

 

Current sources of funding

 

                1) Horizon2020: SPIDIA4P-SPIDIA for Personalized Medicine – Standardisation of generic Pre-analytical procedures for Invitro Diagnostics-G.A. n. 733112

                2) Ministero della Salute/Regione Toscana: Circulating cell-free biomarkers in the diagnosis and follow-up of differentiated thyroid cancer- Project Code RF-2011-02352294

                3) Regione Toscana: FAS-OMITERC-Applicazione degli OMIcs dalla biopsia solida alla biopsia liquida per una TERapia personalizzata del Cancro- CUPD76D16000180002

 

 

10 best publications of the last 5 years

 

Mutational analysis of single circulating tumor cells by next generation sequencing in metastatic breast cancer.

De Luca F, Rotunno G, Salvianti F, Galardi F, Pestrin M, Gabellini S, Simi L, Mancini I, Vannucchi AM, Pazzagli M, Di Leo A, Pinzani P

ONCOTARGET 2016 Mar;

 

Prevalence and number of circulating tumour cells and microemboli at diagnosis of advanced NSCLC.

Mascalchi M, Falchini M, Maddau C, Salvianti F, Nistri M, Bertelli E, Sali L, Zuccherelli S, Vella A, Matucci M, Voltolini L, Pegna AL,

Luconi M, Pinzani P, Pazzagli M

J CANCER RES CLIN 2016 Jan; 142: 195-200

 

Single circulating tumor cell sequencing as an advanced tool in cancer management.

Salvianti F, Pazzagli M, Pinzani P

EXPERT REV MOL DIAGN 2016; 16: 51-63

 

Feasibility of a workflow for the molecular characterization of single cells by next generation sequencing.

Salvianti F, Rotunno G, Galardi F, De Luca F, Pestrin M, Vannucchi AM, Di Leo A, Pazzagli M, Pinzani P

Biomol Detect Quantif 2015 Sep; 5: 23-9

 

Heterogeneity of PIK3CA mutational status at the single cell level in circulating tumor cells from metastatic breast cancer patients.

Pestrin M, Salvianti F, Galardi F, De Luca F, Turner N, Malorni L, Pazzagli M, Di Leo A, Pinzani P

MOL ONCOL 2015 Apr; 9: 749-57

 

 

Tumor-Related Methylated Cell-Free DNA and Circulating Tumor Cells in Melanoma.

Salvianti F, Orlando C, Massi D, De Giorgi V, Grazzini M, Pazzagli M, Pinzani P

Front Mol Biosci 2015; 2: 76

 

Circulating cell-free DNA in cancer.

Pinzani P, Salvianti F, Orlando C, Pazzagli M

Methods Mol Biol 2014; 1160: 133-45

 

SPIDIA-RNA: second external quality assessment for the pre-analytical phase of blood samples used for RNA based analyses.

Malentacchi F, Pazzagli M, Simi L, Orlando C, Wyrich R, Günther K, Verderio P, Pizzamiglio S, Ciniselli CM, Zhang H, Korenková V, Rainen L, Bar T, Kubista M, Gelmini S.

PLoS One. 2014 Nov 10;9(11):e112293. doi: 10.1371/journal.pone.0112293. eCollection 2014

 

Multiparametric analysis of cell-free DNA in melanoma patients.

Salvianti F, Pinzani P, Verderio P, Ciniselli CM, Massi D, De Giorgi V, Grazzini M, Pazzagli M, Orlando C.

PLoS One. 2012;7(11):e49843. doi: 10.1371/journal.pone.0049843. Epub 2012 Nov 27.

 

SPIDIA-RNA: first external quality assessment for the pre-analytical phase of blood samples used for RNA based analyses.

Pazzagli M, Malentacchi F, Simi L, Orlando C, Wyrich R, Günther K, Hartmann CC, Verderio P, Pizzamiglio S, Ciniselli CM, Tichopad A, Kubista M, Gelmini S.

Methods. 2013 Jan;59(1):20-31. doi: 10.1016/j.ymeth.2012.10.007. Epub 2012 Oct 27.

 

 

Previous research experiences

 

Chemiluminescence and Bioluminescence

Immunoassay and luminescence assay in Endocrinology

Antioxidant capacity

Quantitative PCR (qPCR) assay

 

 

 

Main scientific contributions

 

- M.P  is an international recognized expert in Clinical Biochemistry, member of several

societies of Laboratory Medicine: SiBioC (Società Italiana di Biochimica Clinica), IFCC (International

Federation of Clinical Chemistry and Laboratory Medicine), EFLM (European Federation of Laboratory

Medicine). He participated to three European collaborative projects: 2001-2002 Coordinator of the Project

FP5 EU "La Vinci-Linnaeus-Da Darwin Initiative"; 2004-2007 Coordinator of the Project FP6 EU "Multi-

National External Quality Assay (EQA) Programmes in Clinical Molecular Diagnostics based on Performance

and Interpretation of PCR assay methods including dissemination and training”; 2008-2013 Member of the

Project FP7 EU “SPIDIA: Standardisation and improvement of generic pre-analytical tools and procedures

for in vitro diagnostics”. Today, he is the Project Leader of the Reference ISO/NP 20091 “Molecular in vitro

diagnostic examinations -- Specifications for pre-examination processes for blood -- Circulating cell free

DNA” and Reference ISO/NP 20185 “Molecular in-vitro diagnostic examinations -- Specifications for preexamination processes for blood -- Genomic DNA”.

 

 

 

Collaborations

Université Laval, Canada

Prof Massimo Mannelli (Dip. SBSC)

Prof.ssa Michaela Luconi (Dip. SBSC)

Prof. Mario  Mascalchi (Dip SBSC)

Prof Enrico Mini (Dip Medicina Sperimentle e Clinica)

Prof.ssa Daniela Massi (Dip Chirurgia e Medicina Translazionale)

Prof Angelo di Leo, Nuovo Ospedali S Stefano Prato

Dr.ssa Cinzia Mazzini AOU Careggi-SOD Oculistica

Prof Nicola Pimpinelli (Dip Chirurgia e Medicina Translazionale)

Prof Francesco Lolli (Dip. SBSC)

 
ultimo aggiornamento: 18-Lug-2017
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