Name of Research Team/Laboratory:
Functional proteomics and cellular biology
Coordinator Alessandra Modesti Full Professor – Applied Biology
Brief Biographical sketch of the Coordinator
Born: December 27, 1959; Education: 1984 B.A. in Pharmacy. University of Florence, Italy; 1991 Ph.D. Biochemistry, University of Florence, Italy; 1993 Ph.D. Biotechnology, Biotechnology Centre, Cranfield Institute of Technology, Cranfield (GB).
The scientific activity and research interests of Alessandra Modesti focus on studying of proteins, particularly as structural characterization of proteins. The initial approach to proteins has been investigated using the classic molecular biology procedure obtaining recombinant bacteria and yeast S.cerevisiae. Great part of her activity during this first period has been turned to the study of site-directed mutagenesis of residues of the two phosphomonohydrolases. A new strategy for the investigation of proteins has been developed. This approach consists of the use and implementation of modern methodological approach like the Proteomic techniques (2dimentional electrophoresis/mass spectrometry) with the goal of obtaining more information into complex mechanics in order to obtain a molecular anatomy of the cells. A comparative analysis of the proteomic alterations observed in various experimental conditions allows to identify the metabolic and cell signalling mechanisms that are mainly influenced by these events. This information is converted into a detailed and realistic description of the mode of action of each event also using bioinformatics techniques. More recently the main research activities carried-out are focused on the study of redox proteomics and the correlation among oxidative stress with inflammation and peripheral signals.
Member of the Scientific Board the Doctorate/Phd Program in Scienze Biomediche
Memeber of the following Scientific Societis A.I.B.G. Associazione Italiana Biologia e Genetica – Member since 2000
Member of editorial board of the following Journals Antioxidant, Frontiers
Francesca Magherini, PhD, 02/02/1968 Associate Professor:
Tania Fiaschi, PhD, 13/09/1965 Associate Professor:
Tania Gamberi, PhD, 03/01/1973 RtdB:
Riccardo Marzocchini, 01/11/1956 RC:
Maria Stio, 13/12/55 RC
Simone Luti, 08/01/1985 Postdoctoral researcher and adjunct professor
Michele Mannelli, PhD student
Rosamaria Militello, PhD student
Current research interest:
Muscle-hormonal communication and range from the study of proteomic and metabolomic variations in subjects who practice physical activity (Alessandra Modesti).
Oxidative stress induced by various physiological or pathological stressors in cellular models as well as in human biological fluids (Alessandra Modesti).
Ovarian cancer: characterization of primary cultures, tumor microenvironment and innovative therapies (Francesca Magherini)
Mechanisms of action/biological targets of metal-based anticancer drugs (Tania Gamberi).
Adiponectin and Its Isoforms in Pathophysiology (Tania Gamberi)
Cancer cachexia in skeletal muscle and adipose tissue (Tania Fiaschi)
Role of cancer microenvironment in cancer cachexia (Tania Fiaschi)
Study of adiponectin in cancer cachexia (Tania Fiaschi)
Role of vitamina D in inflammatory bowel disease (Maria Stio)
Study of the different expression of LMW-PTP and of Acylphosphatase in healthy and tumour tissues (Riccardo Marzocchini).
Key words – Miokine, inflammation, oxidative stress, proteomics, adiponectin, cancer cachexia, skeletal muscle, adipose tissue, ovarian cancer, metal-bases anticancer drug
Current/recent sources of funding
2011 AIRC progetto IG numero 12085
2013 – Rif. n. 2013.0504 Ente Cassa di Risparmio di Firenze
2013 - Agroils BIOM.CR14MODECTAG13
2014 Rif. N. 2014. 0102 Ente Cassa di Risparmio di Firenze
2015 AIRC progetto IG numero 19650
2015 ITT 2015
2016-AIRC Project code 19650
Finanziamenti di Ateneo ex 60%
10 best pubblications of the last 5 years
Luti S, Fiaschi T, Magherini F, Modesti PA, Piomboni P, Governini L, Luddi A, Amoresano A, Illiano A, Pinto G, Modesti A, Gamberi T. “Follicular microenvironment: Oxidative stress and adiponectin correlated with steroids hormones in women undergoing in vitro fertilization”. Mol Reprod Dev. 2020 Dec 18. doi: 10.1002/mrd.23447.
Luti S, Fiaschi T, Magherini F, Modesti PA, Piomboni P, Governini L, Luddi A, Amoresano A, Illiano A, Pinto G, Modesti A, Gamberi T. “Relationship between the metabolic and lipid profile in follicular fluid of women undergoing in vitro fertilization”. Mol Reprod Dev. 2020 Sep;87(9):986-997. doi: 10.1002/mrd.23415
Landini I, Massai L, Cirri D, Gamberi T, Paoli P, Messori L, Mini E, Nobili S. “Structure-activity relationships in a series of auranofin analogues showing remarkable antiproliferative properties”. J Inorg Biochem. 2020 Jul; 208:111079. doi: 10.1016/j.jinorgbio.2020.111079.
Domazetovic V, Iantomasi T, Bonanomi AG, Stio M. "Vitamin D regulates claudin-2 and claudin-4 expression in active ulcerative colitis by p-Stat-6 and Smad-7 signaling. Int J Colorectal Dis. 2020 Jul;35(7):1231-1242. doi: 10.1007/s00384-020-03576-0
Magherini F, Fiaschi T, Marzocchini R, Mannelli M, Gamberi T, Modesti PA, Modesti A. “Oxidative stress in exercise training: the involvement of inflammation and peripheral signals”. Free Radic Res. 2019 Dec;53(11-12):1155-1165. doi: 10.1080/10715762.2019.1697438.
Mannelli M, Gamberi T, Magherini F, Fiaschi T. “The Adipokines in Cancer Cachexia. Int J Mol Sci. 2020 Jul 9;21(14):4860. doi: 10.3390/ijms21144860.
Gorini G, Gamberi T, Fiaschi T, Mannelli M, Modesti A, Magherini F. “Irreversible plasma and muscle protein oxidation and physical exercise”. Free Radic Res. 2019 Feb;53(2):126-138. doi: 10.1080/10715762.2018.1542141.
Gamberi T, Magherini F, Mannelli M, Chrisam M, Cescon M, Castagnaro S, Modesti A, Braghetta P, Fiaschi T.” Role of adiponectin in the metabolism of skeletal muscles in collagen VI-related myopathies”. J Mol Med (Berl). 2019 Jun;97(6):793-801. doi: 10.1007/s00109-019-01766-0.
Magherini F, Fiaschi T, Valocchia E, Becatti M, Pratesi A, Marzo T, Massai L, Gabbiani C, Landini I, Nobili S, Mini E, Messori L, Modesti A, Gamberi T. "Antiproliferative effects of two gold(I)-N-heterocyclic carbene complexes in A2780 human ovarian cancer cells: a comparative proteomic study”. Oncotarget. 2018 Jun 15;9(46):28042-28068. doi: 10.18632/oncotarget.25556.
Lori G, Gamberi T, Paoli P, Caselli A, Pranzini E, Marzocchini R, Modesti A, Raugei G. LMW-PTP modulates glucose metabolism in cancer cells. Biochim Biophys Acta Gen Subj. 2018 Dec;1862(12):2533-2544. doi: 10.1016/j.bbagen.2018.08.003. Epub 2018 Aug 4. PMID: 30251652.
Thomas Hawke, Department of Pathology & Molecular Medicine, McMaster University, Hamilton, ON, Canada
Merry L. Lindsey, Ph.D. Director, Mississippi Center for Heart Research, Department of Physiology and Biophysics. University of Mississippi Medical Center. Jackson, MS
Luca Bini. PhD. Professor DIPARTIMENTO DI SCIENZE DELLA VITA. University of Pisa, Italia
Angela Amoresano, PhD, Professor Dipartimento di Scienze chimiche, Università di Napoli Federico II, Italia
Paolo Bonaldo, PhD, Dipartimento di Medicina Molecolare, Università di Padova, Italia
Paola Braghetta, PhD, Dipartimento di Medicina Molecolare, Università di Padova, Italia
Paola Piomboni, PhD Dipartimento di Medicina Molecolare e dello Sviluppo Università di Siena
Daniela Caporossi PhD Dipartimento di Scienze Motorie, Umane e della Salute, Università di Roma “Foro Italico”