Coordinator of Research Team
Name Alessandra Modesti
Position Associate Professor
Name of Research Team/Laboratory Laboratorio di proteomica applicata ai sistemi biologici
telephone number 0039 055 2751237
Brief biographical sketch of the Coordinator (date of birth, degrees and Universities where they have been obtained)
Born: December 27, 1959; Viareggio (LU), Italy
1984 B.A. in Pharmacy. University of Florence, Italy
1987 post doctoral training at the European Molecular Biology Laboratory (EMBL) in Heidelberg.
1991 Ph.D. Biochemistry, University of Florence, Italy
1993 Ph.D. Biotechnology, Biotechnology Centre, Cranfield Institute of Technology, Cranfield (GB).
1994 post doctoral training at the Instituto de Investigaciones Biomedica IIB of Madrid (Spain).
1985-1986 Fellowship Menarini Industrie Farmaceutiche Riunite Srl, Florence, Italy
1987-1989 Post-graduate fellowship program, CEE
2000 - Associate Professor, Applied Biology – Faculty of Medicine - University of Florence
1995 - 2000 Research Scientist (permanent position), Dept Scienze Biochimiche, University of Florence, Italy
1990 – 1995 Technician - Dept Scienze Biochimiche, University of Florence, Italy
Journal of proteome research
Member of the scientific board of the Doctorate/PhD Program in
Università degli Studi di FIRENZE Titolo: "SCIENZE BIOMEDICHE"
Member of the following Scientific Societies
1) Italian Human Proteome Organization (IHUPO) – Founder and member since 2004.
2) Italian Proteomics Association (ItPA) – Member since 2007 and secretary since 2010
3) A.I.B.G. Associazione Italiana Biologia e Genetica – Member since 2000
Member of the editorial board of the following Journals
1) Tania Gamberi, PhD, 03/01/1973
2) Tania Fiaschi, PhD, 13/09/1965
3) Francesca Magherini, PhD, 02/02/1968
Current research interests
The research interests of Prof. Alessandra Modesti focus on the study of proteins. The initial approach to proteins has been investigated using the classic biochemical and molecular biology procedure obtaining recombinant proteins. Great part of her activity has been turned to investigation of proteins by proteomic technology with the goal of obtaining more information into complex mechanics in order to obtain a molecular anatomy of the cells. Careful and comparative analysis of proteomic alterations observed under various experimental conditions allow to identify those parts of the cells are primarily affected by investigational different events. During the last 6 years the research activity of AM has focused on gold compounds as anticancer agents in collaboration with the research group of Luigi Messori from the Department of chemistry “Ugo Schiff”, University of Florence. The outcomes obtained in this research area are listed below:
1 – Proteomic analysis to study the Biochemical mechanisms of cytotoxic gold compounds.
2 - Proteasome as a target for gold compound in cancer cells showing resistance to cis-platinum.
3 - Alterations in the glycolytic pathway revealed for gold compound Aubipyc by proteomic analysis.
4 - Bioinformatic analysis of proteomic data.
5 - The antiproliferative effects of auranofin is due to an inhibition of mitochondrial respiration.
More recently in collaboration with prof Merry Lyndsey from Mississippi and Prof. P.A. Modesti from the university or Florence she is interested in combining their expertises in proteomics and bioinformatics, as well as cardiac disease pathologies, to better understand the mechanisms involved in cardiac remodeling after myocardial infarction. In particular, focusing on inflammatory and fibrotic responses to myocardial infarction will provide us with the experimental platform on which to build collaborative studies involving proteomic analysis of plasma and tissue samples. Using both mouse models and human samples, our translational collaboration will identify novel targets for designing therapies to combat adverse cardiac remodeling.
Current / recent sources of funding
1) 2011 AIRC progetto IG numero 12085
2) 2013 – Rif. n. 2013.0504 Ente Cassa di Risparmio di Firenze
3) 2013 - Agroils BIOM.CR14MODECTAG13
4) 2014 Rif. N. 2014. 0102 Ente Cassa di Risparmio di Firenze
5) 2015 AIRC progetto IG numero 19650
6) 2015 ITT 2015
10 best publications of the last 5 years
- Carpentieri A, Gamberi T, Modesti A, Amoresano A, Colombini B, Nocella M, Bagni MA, Fiaschi T, Barolo L, Gulisano M, Magherini F. Profiling Carbonylated Proteins in Heart and Skeletal Muscle Mitochondria from Trained and Untrained Mice. J Proteome Res. 2016 Oct 7;15(10):3666-3678.
- Gamberi T, Magherini F, Fiaschi T, Valocchia E, Raugei G, Modesti P.A., Messori L and Modesti A. Systems Biology of the Proteomic Analysis of Cytotoxic Gold Compounds in A2780 Ovarian Cancer Cell Line: A Network Analysis. J Bioinform Proteom. 2016, 1(1): 004.
- Gamberi T, Modesti A, Magherini F, D'Souza DM, Hawke T, Fiaschi T. Activation of autophagy by globular adiponectin is required for muscle differentiation. Biochim Biophys Acta. 2016 Jan 26;1863(4):694-702. doi: 10.1016/j.bbamcr.2016.01.016.
- Gamberi T, Fiaschi T, Modesti A, Massai L, Messori L, Balzi M, Magherini F. Evidence that the antiproliferative effects of auranofin in Saccharomyces cerevisiae arise from inhibition of mitochondrial respiration. Int J Biochem Cell Biol. 2015 Aug;65:61-71. doi: 10.1016/j.biocel.2015.05.016. Epub 2015 May 27.
- Deleon-Pennell KY, Altara R, Yabluchanskiy A, Modesti A, Lindsey ML. The circular relationship between matrix metalloproteinase-9 and inflammation following myocardial infarction. IUBMB Life. 2015 Aug;67(8):611-8. doi: 10.1002/iub.1408. Epub 2015 Aug 13.
- Fiaschi T, Magherini F, Gamberi T, Lucchese G, Faggian G, Modesti A, Modesti PA. Hyperglycemia and angiotensin II cooperate to enhance collagen I deposition by cardiac fibroblasts through a ROS-STAT3-dependent mechanism. Biochim Biophys Acta. 2014 Nov;1843(11):2603-10. doi: 10.1016/j.bbamcr.2014.07.009. Epub 2014 Jul 27.
- Pietrovito L, Cano-Cortés V, Gamberi T, Magherini F, Bianchi L, Bini L, Sánchez-Martín RM, Fasano M, Modesti A. Cellular response to empty and palladium-conjugated amino-polystyrene nanospheres uptake: A proteomic study. Proteomics. 2014 Oct 30. doi: 10.1002/pmic.201300423.
- Tian Y, Koganti T, Yao Z, Cannon P, Shah P, Pietrovito L, Modesti A, Aiyetan P, DeLeon-Pennell K, Ma Y, Halade GV, Hicks C, Zhang H, Lindsey ML. Characterization of cardiac extracellular proteome and membrane topology using glycoproteomics. Proteomics Clin Appl. 2014 Jun 11
- Gamberi T, Massai L, Magherini F, Landini I, Fiaschi T, Scaletti F, Gabbiani C, Bianchi L, Bini L, Nobili S, Perrone G, Mini E, Messori L, Modesti A. Proteomic analysis of A2780/S ovarian cancer cell response to the cytotoxic organogold(III) compound Aubipyc. J Proteomics. 2014 May 30;103:103-20.
- Modesti A.,Alberio T.,Gamberi T.,Fasano M.,Magherini F.,Fiaschi T.,Balzi M.,Lindsey M.L. and Modesti P.A. Network Biology Analysis of the Human Abdominal Aortic Aneurysm Plasma Proteome. Austin J Proteomics Bioinform & Genomics. 2014;1(1): 10.
Previous research experiences
EUROPEAN MOLECULAR BIOLOGY LABORATORY DI HEIDELBERG (GERMANIA)
Cranfield Institute of Technology (CIT) (Inghilterra)
Ricerca Instituto de Investigaciones Biomedica IIB di Madrid (Spagna)
Main scientific contributions
Thomas Hawke, Department of Pathology & Molecular Medicine, McMaster University, Hamilton, ON, Canada
Merry L. Lindsey, Ph.D. Director, Mississippi Center for Heart Research, Department of Physiology and Biophysics. University of Mississippi Medical Center. Jackson, MS
Luca Bini. PhD. Professor DIPARTIMENTO DI SCIENZE DELLA VITA. University of Pisa, Italia
Angela Amoresano, PhD, Professor Dipartimento di Scienze chimiche, Università di Napoli Federico II, Italia
Paolo Bonaldo, PhD, Dipartimento di Medicina Molecolare, Università di Padova, Italia
Paola Braghetta, PhD, Dipartimento di Medicina Molecolare, Università di Padova, Italia
Other relevant information